Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. e. Customer Transformation Hub. The VICTOR Nivo is a HTRF certified microplate reader. CLARIOstar is equipped with a specific optical device which enables the measurement of. certified for use with HTRF. 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF ® pre-mixed antibodies. best fluorescence polarization plate reader on the market. • Clone your plate data for multiple data reduction optionsExpanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. In addition, stacker. 96-well culture plateFOR HTRF CERTIFIED READER. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Read & analyse. Read & analyse. No washing steps are required. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Dual-Luciferase® (DLR™) gene assays. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:320/40 HTRF Eu cryptate 665/7uv Wide Filter 0. Evaluation of the performance of the Spark™ multimode reader for far red fluorescence emission. As shown in Fig. This protocol. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Lysates are then transferred to the assay plate for the detection of total-NFkB by HTRF. Plus . + 4 µL HTRF ® pre-mixed antibodies . Introduction. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. These convert photons generated by fluorescence or. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. EM1 616/10 nm EM2 665/10 nm Certified for use with Cisbio’s HTRF Europium Cryptate reagents. Incubate overnight at RT. HTRF is a FRET-based technology that emerged from the Jean-Marie Lehn ’s Nobel prize-winning work on rare earth complexes. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. MUTANT HTT STANDARD WORKING SOLUTIONSLysis buffer 4X for two-plate assay protocol on suspension cells Determine the amount of lysis buffer needed for the experiment. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. Plate 25 μL of cells in. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For more ® information about HTRF compatible readers and for set-up recommendations, please. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYDetection. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. The VICTOR Nivo is a HTRF certified microplate reader. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. FOR HTRF CERTIFIED READER For more information about HTRF. Taking these considerations into account, Cisbio’s 96w low volume and PKI’s 96w & 384w microplates have been carefully designed to provide the best signal detection environment possible to all HTRF assays. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. It guarantees high level of cAMP accumulation in the cells. The signals are produced by, converted by, or transmitted through the. Envision Nexus. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. * For HTRF microplate recommendations, please visit . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. It enables you to gain insights you couldn’t achieve with detection measurements alone. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". AlphaLISA SureFire Ultra results are expressed as “Alpha counts”, and HTRF results were expressed as DeltaF, calculated according to CisBio instructions. Transfer 5 µL of cell supernatant from the 96-well plate to a 384-small volume (SV) white detection plate . FOR HTRF CERTIFIED READER For. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. n HTRF plate validated (QC with reference HTRF assays) n Low to high volumes (10 µL to 350 µL) n Can be used with all HTRF compatible readers n For cell-based or biochemical assays Trying is Believing! Plate Characteristics HTRF® 96 - well low volume plate CulturPlateTM 96 - well Microplate CulturPlate TM 384 - well Microplate ProxiPlateTM 384 - Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. ratio, high sensitivity, wide dynamic range, superior stability, and excellent flexibility. For more information about HTRF. Conclusion. • User-upgradable microplate reader in under 2 minutes • Array of detection cartridge applications at your fingertips. NON-SPECIFIC BINDING TOTAL BINDINGCover the plate with a plate sealer. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Revvity. Lysates are then transferred to the assay plate for the detection of phospho- CHK2 (Thr 68) by HTRF. It utilises an ultra-fast UV/vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes. FOR HTRF CERTIFIED READER. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For more information about HTRF. The measurement of HTRF assays is predominantly performed on microplate readers. Seal the plate and incubate 3 hours at RT . Make sure the setup for Eu Cryptate is used. Read & analyse. For more information about HTRF. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. Plates can be loaded manually or plates can be loaded using 20 or 50-plate free access magazines which fit in a stacker. Remove the plate seal and read on an HTRF compatible reader. For more information about microplate recommendations, please visit our website STORAGE AND STABILITY• HTRF®-Certified Reader. Assay setup for a 384-well low-volume plate. Incubate 4h at RT. Please note that this kit use a Terbium donor crypate, make sure to set up your reader for Terbium cryptate. Lysates are ®then transferred to the assay plate for the detection of phospho- Rab10 (Thr73) by HTRF reagents. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. HTRF reagent concentrations have been set for optimal assay performances. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. It is typically used to determine the concentration of nucleic acids and proteins. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The HTRF ratio obtained represents the total binding. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. 7ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. FOR HTRF CERTIFIED READER For more. For more information about HTRF. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the market with all major detection technologies including Alpha and certification for HTRF® technology plus software designed for 21 CFR Part 11. • HTRF®-Certified Reader. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. com HTRF Microplate Readers. SV detection plate. It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background. 1 – 1. culture plate. HTRF is compatible with a large range of available microplate readers. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates to a low volume detection plate (either HTRF 384-lv or 96-lv plate) before the addition of HTRF Phospho-Rb (Ser780) detection reagents. The Bradford assay was successfully performed on the BMG LABTECH microplate reader. FOR HTRF CERTIFIED. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. But sometimes these parameters are not sufficient enough. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). 96-well culture plateFOR HTRF CERTIFIED READER. Read & analyse. 3-in-1 absorbance using a variety of sample formats, including microplates. It is used as a positive control for Gs applications ( biological models validation,Detection of Total CDK12 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. The CulturPlate is PerkinElmer’s microplate designed for cellular assays, tissue-culture treated and sterilized by gamma-irradiation to facilitate adherent cell growth. Preconfigured HTRF. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. 4. Nephelometers are dedicated instruments that can only detect scattering and are only available as standalone plate readers. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-3 (Ser473) by HTRF. FOR HTRF CERTIFIED READER. There are multiple factors to consider including: detection type, additional. Note that any dilution or improper use of d2 and cryptate Tb-detection reagents will impair the assay quality. The PHERAstar ® FSX and CLARIOstar ® microplate readers offer the best possible performance for AlphaScreen ®, AlphaLISA ®, and AlphaPlex™ assays. → Cover the plate with a plate sealer. + 4 µL HTRF pre-mixed antibodies. The plate was covered and incubated for one hour at room temperature. Make sure the setup for Tb Cryptate is used. Its. Conclusion. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight at RT * * Depending on cell lines used, volume of lysis should be optimized. A gradient of signal is apparent prior to plate reader optimization (a) causing a %CV of 9. SERIAL DILUTIONS . Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. FOR HTRF CERTIFIED READER For. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The reader only allows high performance for the used HTRF measurement when the HTRF is run with white plates. Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Microplate reader assays requiring infrared (IR) or. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. 25,000 human Jurkat cells were plated in 96 well plates. • ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Plate cells Stimulate cells 3. Concentrate on running your assays instead of running your instrument: the optic modules contain all the filters, dichroics, and more needed to run your assay. (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based). • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersFOR HTRF CERTIFIED READER. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Because of its homogeneous and fast nature, the assay is a preferred method to determine the protein concentration of samples. + 4 µL HTRF ® pre-mixed antibodies . FOR HTRF CERTIFIED READER. For more information about set-up and compatible HTRF ® readers, please visit our website at: ® pre-mixed antibodies TWO- PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Set up your reader for Eu3+ Cryptate and read the fluorescence emission at two different wavelengths (665nm and 620nm) on a compatible HTRF® reader**. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Lysates are then transferred to the assay plate for the detection of total-p53 by HTRF. -CERTIFIED READER IS NEEDED. For more information. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Plate 25 μL of cells in. Make sure to use the set-up for Eu Cryptate. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-2 (Ser473) by HTRF. Lyse cells Read on an HTRF compatible reader . -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Thanks to HTRF signal stability, the assay plate can be used to check several instruments, or different reader. The ratio between control lysate signal / non-specific signal should be greater than 2. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. CLARIOstar is equipped with a specific optical device which enables the measurement of. Remove the plate seal and read on an HTRF compatible reader. It can also be further streamlinedFOR HTRF CERTIFIED READER. BMG LABTECH’s UV-laser not only allows for faster read times in europium TRF assays, but it also greatly enhances performance in terbium-based TRF assays. at room temperature STEP 4 Remove the plate sealer and read on an HTRF ® compatible reader. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. For more ® information about HTRF compatible readers and for set-up recommendations. In particular, these parameters should be entered as below. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The plate is set up for reading and images are acquired according to specified parameters. These plates can be read on all HTRF certified readers, thus making their implementation in. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Make sure the setup for Tb Cryptate is used. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The optical systems use two scanningHighly compatible plates for HTRF. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Plate 25 μL of cells in. Lysates are then transferred to the assay plate for the detection of phospho-Tie2 (Tyr992) by HTRF. For more information about set-up and compatible HTRF® readers,The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. These are the different kinetic modes on BMG LABTECH microplate readers. HTRF 96 TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: 3h * * Depending on cell lines used, volume of lysis should be optimized. Even if not listed, some. Figure 1. * For HTRF microplate recommendations, please visit . After an overnight incubation, the plate is washed 3 times. The FLUOstar Omega is the ideal plate reader for life science applications. Your lab might even have one already. 1. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. HTRF is a proprietary time-resolved fluorescence technology that overcomesThe company offers different HTRF-certified, multidetection microplate readers such as the FLUOstar Omega, POLARstar Omega, CLARIOstar and PHERAstar. 96-well culture plate+ 4 µL HTRF pre-mixed antibodies. Read & analyse. The optimized well design and high quality plastics provides low background and crosstalk. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf certifiedLysates are then transferred ®to the assay plate for the detection of Total CHK2 by HTRF reagents. FOR HTRF CERTIFIED READER . These plates can be read on all HTRF certified readers, thus making their implementation in. The VICTOR Nivo is a HTRF certified microplate reader. As shown in Fig. February 23, 2010, WINOOSKI VT, USA - The Synergy TM H4 Hybrid Microplate Reader from BioTek Instruments is now HTRF® certified. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm:. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. 6% for 1,408 wells and a Z’ value of 0. PLATE READER INTRODUCTION Selecting the right plate reader for your lab can be challenging. Assay detection requires a microplate reader with. Step 1 Step 2 Step 3 Step 4 Step 5. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. This is thanks to a dedicated Alpha Technology laser which outperforms any xenon-based light source by exciting donors at 680 nm. Phospho-Rb (Ser780) two-plate assay protocol. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Remove the plate sealer and read on an HTRF® compatible reader . Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". 5 µL. 2 Locking the Plate Carrier Upon closing the software or pushing the plate carrier in/out button for more than three seconds (figure 10), the plate carrier will move into the respective lock position. FOR HTRF CERTIFIED READER . For more information about microplate recommendations, please visit our website • Store the kit at -16°C. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. FOR HTRF CERTIFIED READER. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. No washing steps are required. Do not pre-mix the d2 and Cryptate solutions prior to dispensing Make sure to use the set-up for Eu Cryptate. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. FlexStation® 3 Multi-Mode Microplate Reader. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Make sure the setup for Tb3+ Cryptate is used. FOR HTRF CERTIFIED READER. Read other about this measurement approach here. Remove the plate seal and read on an HTRF compatible reader. • Select Excitation Filter 320x40 HTRF Eu cryptate* • Select Excitation Optic Empty • Select Emission Filter 495xm10uv • Select Emission Optic 2 – FPThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. HTRF Setup. The HTRF cellular assays were found to be at least 16-fold more sensitive than the Western Blot. FOR HTRF CERTIFIED READER For. 0. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. You can verify your reader’s capacity to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. HTRF combines time-resolved fresh detection with FRET and is mainly used for drug screening in high-throughput. HTRF Signal + 5 hours @ RT or . Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. Other technologies such as imaging. Fluorescence intensity – Fluorescence intensity is one of the most. S. LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. FOR HTRF CERTIFIED READER. 5 hours before 3 more washing steps. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR HTRF CERTIFIED READER. This protocol gives the cells viability and confluence to be monitored. HTRF Signal + 16 µL 4 µL Standard of pre-mixed IL8 Antibodies or Sample ADD READ ANALYSE Incubate 1 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. Finding a plate reader that offers compatibility with microplates sold by multiple leading vendors can protect both the long-term cost and the performance of assays. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detectionThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Incubate 2 h at RT. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Place the imaging filter cube on top of the LED cube you installed, and use the hex wrench to screw it to the LED cube. At Cisbio we provide microplates that help you discover better. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. It can also be further streamlined toFOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. Plate 25 μL of cells in. In particular, these parameters should be entered as defined in the table below Setup Top filter cube EX 330 / LUM (block #18) EM 620 / 665 / LUM (block #41) Light source LaserDetect flash, glow, Dual Luciferase ® Reporter and BRET assays. This protocol gives the cells viability and confluence to be monitored. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READER For more information about HTRF. FOR READING, AN HTRF ®-CERTIFIED READER IS NEEDED. MATERIALS PROVIDED: KIT . They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. For more information about HTRF. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. The plate reader. Plate 1 plate HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. The adapterResults summary for HTRF IP1 standard curve. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. This protocol gives the cells viability and confluence to be monitored. Remove the plate seal and read on an HTRF compatible reader. For HTRF certified reader For more information about HTRF® compatible readers and for set- up recommendations, please visit our website. The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Discover the features and benefits of the VICTOR Nivo multimode plate reader, the smallest and most versatile instrument for life science applications. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. • Low volume (20µl) • Compatible with all HTRF readers • For cell-based or biochemical assays Trying is believing. Typical applications The HTRF cartridge provides optimal performance for key applications. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). Download Setup. $1,024. Neocarzinostatin dose-response on Jurkat cells. This protocol gives the cells viability and confluence to be monitored. For instance, when assay volumes are. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The optional HTRF-certified filter-based module may be. Multimode plate readers include, at the very least, some combination of absorbance (typically covering the UV/visible range), fluorescence and luminescence. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. Read & analyse. 5 EXAMPLE OF MAP PLATE . CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. This protocol, HTS designed, allows. PURCHASE SEPARATELY . At its core, Synergy H1 is a monochromator-based multi-mode microplate reader with a quadruple grating architecture for robust performance and flexibility. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:FOR HTRF CERTIFIED READER. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. $352. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). + 4 µL HTRF ® pre-mixed antibo dies 2. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. 10 µL Standard or Sample. The plate was covered and incubated for two hours at room temperature. PerkinElmer. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large range of available microplate readers and certainly the detection system already in your lab. The kit includes everything you need: reagents, buffers and a microplate. Make sure the setup for Tb Cryptate is used. NC1286225. + 4 µL HTRF pre-mixed antibodies . Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for excellence. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Make sure the setup for Tb Cryptate is used. If not, our scientific team will help you choose the instrument that best suits your needs and budget. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). (2) Forskolin activates the adenylyl cyclase enzyme and increases the intracellular level of cAMP. support such assays and even feature endorsements from reagent suppliers saying the reader is “HTRF compatible,” “LanthaScreen Certified Plus,” Transcreener Validated” or. For a list of HTRF-compatible readers and set-up recommendations, please visit . PHERAstar FSX Powerful and most sensitive HTS plate reader . The 2 plate protocol involves culturing cells in a 96-well plate before lysis then transferring lysates to a 384-well low volume detection plate before adding Phospho-ERK (Thr202/Tyr204) HTRF detection reagents. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Smallest and most affordable multimode plate reader in the industry. FOR HTRF CERTIFIED READER. Tecan Spark multimode microplate reader, with fluorescence Fusion Optics™ meets these challenges and offers everything you need to develop and run advanced biochemical and cell-based fluorescence assays. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. best fluorescence polarization plate reader on the market. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. 5 EXAMPLE OF MAP PLATE . Incubate 4h at room temperature. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. The ratio for each well must be calculated individually. * For HTRF microplate recommendations, please visit binding of the fluorescent ligand to the receptor, TR-FRET occurs and is measured with an HTRF-certified microplate reader. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 68. See the Cytation 5 Operator’s Manual for more information and instructions. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. Cells in each image are identified by SoftMax Pro Software and cell-by-cellHTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. 9. FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER.